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Vol. 10 (2007 year), No. 4
Kuchina Yu.A., Shoshina E.V., Dubrovin S.Yu., Putintsev N.M., Konovalova I.N., Vasilevsky P.B.
The electrochemical method of enzymatic protein hydrolysates' production out of hydrobionts used for microbiological nutrient media
The paper considers the kinetics of alkaline hydrolysis of N-acetylglucosamine and D-glucosamine depending on temperature and reagents' concentration. The mathematical model of hydrolysis has been proposed, velocity constants and the energy of reaction activation have been calculated.
(in Russian, стр.5, fig. 1, tables. 4, ref 8, Adobe PDF, Adobe PDF 0 Kb)
Vol. 15 (2012 year), No. 1
Zotova K.V., Kuchina Yu.A., Shironina A.Yu., Novikov V.Yu., Derkach S.R.
Influence of enzymic hydrolysis duration on properties of protein hydrolysates
Physicochemical properties of enzymic protein hydrolysates have been investigated. It has been found out that the viscosity of the hydrolysates is decreasing with increase of the hydrolysis time. Enzymic hydrolysis being conducted during 60 minutes results the accumulation of protein fractions with molecular weight about 100 kDa in the hydrolysate.
(in Russian, стр.5, fig. 5, tables. 0, ref 10, Adobe PDF, Adobe PDF 0 Kb)
Vol. 15 (2012 year), No. 1
Kuchina Yu.A., Dolgopyatova N.V., Novikov V.Yu., Sagaidachny V.A., Morozov N.N.
Instrumental methods of determining the degree of chitin deacetylation
The overview of methods for determination the chitin and chitosan deacetylation degree has been carried out. The optical density of chitosan samples with different molecular weight in the infrared has been defined. The comparative results of measuring chitin deacetylation degree by the method of infrared spectroscopy and using registration of chitin acid hydrolysis product by the liquid chromatography method have been given.
(in Russian, стр.7, fig. 6, tables. 2, ref 16, Adobe PDF, Adobe PDF 0 Kb)
Vol. 16 (2013 year), No. 3
Reut K.V., Dolgopyatova N.V., Novikov V.Yu., Putintsev N.M., Konovalova I.N., Kuchina Yu.A.
Optical activity and spectrophotometric characteristics of chitosan solutions obtained from the Kamchatka crab and the Arctic shrimp
The size of specific optical rotation of chitosan solutions has been shown to depend on nucleophilicity of hydrolyzing agent, it grows in absolute value with increase of quantities of trailer glycosidic centers in chitosan solution; the chitosan deacetylation degree practically does not influence on the size of specific optical rotation. For chitosan from chitin of the Kamchatka crab and the Arctic shrimp identical laws of influence of molecular weight and deacetylation degree on the size of specific optical rotation have been obtained. Influence of phosphoric acid on conformational transformations trailer glycosidic centres of chitosan molecules has been defined.
(in Russian, стр.6, fig. 6, tables. 2, ref 12, Adobe PDF, Adobe PDF 0 Kb)