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Vol. 19 (2016 year), No. 3, DOI: 10.21443/1560-9278-2016-3

Kuranova L. K., Zhivlyantseva Yu. V., Grokhovsky V. A.
The study of biological value of peptone derived from secondary raw fish

The possibility of using secondary fish raw materials in particular musculoskeletal waste from filleting the cod species of fish as raw material base for production of food peptones has been studied. The content of water, fat, protein substances, chloride sodium in the raw material and the product have been determined by standard methods. Amino-acid composition has been determined by high effective liquid chromatography. The biological value of peptone and the balance of essential amino acids have been calculated. It has been established that waste from the cod filleting (the humerus with excess flesh) contains 18.95 % protein and a slight (0.15 %) amount of fat. The models of peptone – fermentative fish hydrolyzate obtained from cod withdrawals – have been researched. Proteolytic activity used in the hydrolysis of enzyme (protosubtilin G3X) has been defined as 560.77 mmol TYR/g. Peptone is an amorphous, fine powder of a light beige color. The product is hygroscopic, readily soluble in water. The chemical and biochemical quality characteristics of enzymatic peptone have been defined. Mass fraction of protein in the derived product is 92.27 %, mass fraction of water – 4.7 %, sodium chloride and 2.6 %, fat – 0.3 %. The total number of amino acids in the product is 94.7 %. Peptone in protein contains all the essential amino acids, the total content of which is 40.8 %. The biological value of the product has been estimated by calculation methods. It has been established that tryptophan is the only limiting amino acid in the protein of peptone, amino-acid score is 66.8 %, the rationality coefficient of the product is 0.42, which characterizes it as a fairly balanced protein product. Peptone obtained from secondary fish raw materials by protein content and its properties corresponds to the category of protein isolates and can be recommended for use in food as complete protein food additives and as protein content in sports nutrition products

(in Russian, стр.8, fig. 4, tables. 1, ref 14, Adobe PDF, Adobe PDF 0 Kb)

Vol. 28 (2025 year), No. 3, DOI: 10.21443/1560-9278-2025-28-3

Zhivlyantseva Yu. V., Kuranova L. K.
Scientific justification of the shelf life of fish protein hydrolysate obtained using the enzyme protosubtilin G3x

Fish protein hydrolysate obtained by enzymatic hydrolysis from recycle fish materials using the enzyme protosubtilin G3x is a protein component in alternative microbiological culture media. The dried form of fish protein hydrolysate (FPH) is more preferable compared to the liquid one due to a longer shelf life, a more convenient method of storage and transportation. The shelf life of the culture medium is one of the main characteristics that the buyer of this product focuses on. The purpose of the work is to scientifically substantiate the shelf life of FPH, which will be used as a basis for the preparation of alternative culture media. Organoleptic, microbiological and physicochemical methods have been used to conduct the research. Three batches of experimental samples of FPH have been prepared and stored in hermetically sealed jars in a dark dry place at a certain temperature and humidity. A scheme for studying fish protein hydrolysate after a certain period of time has been developed taking into account the reserve factor. All batches of fish protein hydrolysate have been tested in accredited laboratories. The content of toxic elements in FPH is: lead – less than 1.0 mg/kg, cadmium – less than 0.2 mg/kg, arsenic – less than 5.0 mg/kg, mercury – 0.5 mg/kg. The sum of nitrosamines (NDMA and NDEA) is less than 0.003 mg/kg, polychlorinated biphenyls – less than 2.0 mg/kg. Pesticide analysis has revealed the presence of hexachlorocyclohexane (alpha-, beta-, gamma-isomers) in quantities of less than 0.02 mg/kg, DDT and its metabolites – less than 0.2 mg/kg. The radionuclides content does not exceed the permissible limit and is: cesium-137 – 4.3 ± 0.1 Bq/kg, strontium-90 – 3.9 ± 0.1 Bq/kg. Microbiological studies have established the absence of coliform bacteria, Staphylococcus aureus, pathogenic salmonella, as well as the absence of indicators of putrefactive spoilage (proteus, mold, yeast). The results of the studies have shown the possibility of storing fish protein hydrolysate for 4 years.

(in Russian, стр.6, fig. 1, tables. 2, ref 5, AdobePDF, AdobePDF 0 Kb)