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On the issue of assessing the main kinetic parameters of enzymatic reactions in obtaining grain hydrolysates
DOI: 10.21443/1560-9278-2025-28-3-329-341
Abstract. Evaluation of the main kinetic parameters and determination of optimal conditions for carrying out enzymatic reactions, selection of enzyme preparations (EP) and multienzyme compositions (MEC) is a topical problem, the solution of which is aimed at optimizing the modification process of different types of grain bran. This is primarily due to the search for ways to improve the efficiency of enzymatic catalysis, the possibility of obtaining grain hydrolysates of a given composition for their further use in order to create new balanced feed and food products of increased nutritional and biological value. Experimental data on the main kinetic parameters of enzymatic hydrolysis under the action of EP trypsin, pepsin, papain on a natural substrate (different types of bran) have been presented. Based on the results of experimental and computational-graphical works, taking into account the accepted assumptions, the "apparent" Michaelis constants and Vmax have been determined, which indirectly indicate different affinities of the studied enzyme preparations to the substrate (bran). The inhibitory activity of aromatic amino acids (tyrosine and tryptophan) in relation to the EP papain and pepsin has been established: at a concentration of 0.6 mg/ml, inhibition is in the range of 90–100 % for different types of bran. The exception is the EP trypsin, in relation to which the inhibitory activity is weakly expressed in both cases. Using the EP papain as an example, the competitive nature of inhibition has been established. High efficiency of MEC (Agrosil Premium + Papain + Agrofit) in action on different types of bran has been revealed. The use of MEC allows to increase the amount of water-soluble protein on average for wheat bran by 3.5 times; rye bran – by 3.1; oat bran – by 2.5; corn bran – by 2.2; buckwheat bran – by 3 times. The gel chromatography method has established the ratio of high-, medium- and low-molecular fractions of proteolysis products.
Printed reference: Vitol I. S., Meleshkina E. P. On the issue of assessing the main kinetic parameters of enzymatic reactions in obtaining grain hydrolysates // Vestnik of MSTU. 2025. V. 28, No 3. P. 329-341.
Electronic reference: Vitol I. S., Meleshkina E. P. On the issue of assessing the main kinetic parameters of enzymatic reactions in obtaining grain hydrolysates // Vestnik of MSTU. 2025. V. 28, No 3. P. 329-341. URL: https://vestnik.mauniver.ru/v28_3_n104/01_Vitol_329-341.pdf.
