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Karpenko D. V., Grishin A. G., Prudnik T. V.
Activation of brewer's yeast by pre-treatment with ultrasound
DOI: 10.21443/1560-9278-2025-28-4/2-602-613
Abstract. Fermentation activity of yeast and intensity of their population development determine technological and economic results of the production cycle in brewing. Ultrasonic treatment of pitching yeast suspension has been carried out for a fixed time at frequency of 40 kHz and power of 30 W before their introduction into nutrient media: depleted (sterile 5 % sucrose solution); complete (beer wort with different concentration of dry substances). Cultivation has been carried out for 4 days at 28–30 °C (on a depleted nutrient medium) and 6 days at 16–18 °C (on a complete one). A nonlinear, but significant effect of preliminary ultrasound treatment on the parameters of nutrient media and yeast populations compared with the control samples, is experimentally revealed. The greatest weight loss of the depleted nutrient medium (25 %) is achieved as a result of ultrasound exposure to pitching yeast for two minutes; an increase in the number of yeast cells on it (approximately 100 %) is observed after a three-minute treatment. The ethanol content in beer wort fermented with yeast after a two-minute treatment has increased by 27 %; after a three-minute treatment, by 11 %. Exposure to ultrasound for 3 minutes provided the greatest increase in the number of yeast cells at the end of their cultivation on a complete nutrient medium (by 153 %). Based on the combined results, ultrasound treatment for three minutes is recognized as the most appropriate; the experiment under these conditions has been repeated twice more, and the obtained data confirm the established patterns. Based on the obtained results, a conclusion is drawn about the potential of using ultrasound treatment in the processes of yeast generation and fermentation of beer wort, and the need for continued research in this area has been noted.
Printed reference: Karpenko D. V., Grishin A. G., Prudnik T. V. Activation of brewer's yeast by pre-treatment with ultrasound // Vestnik of MSTU. 2025. V. 28, No 4. P. 602-613.
Electronic reference: Karpenko D. V., Grishin A. G., Prudnik T. V. Activation of brewer's yeast by pre-treatment with ultrasound // Vestnik of MSTU. 2025. V. 28, No 4. P. 602-613. URL: https://vestnik.mauniver.ru/v28_4_2_n106/03_Karpenko_602-613.pdf.
